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- Antigen structure
- References [4]
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- Flow cytometry [1]
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- Product number
- MAB1258-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17E/IL-25 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-17E in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse IL-17E and no cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, or rhIL-17F.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9H293- Isotype
- IgG
- Antibody clone number
- 182203
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-25 or IL-17E Protects against High-Fat Diet-Induced Hepatic Steatosis in Mice Dependent upon IL-13 Activation of STAT6.
Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.
IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
Wang AJ, Yang Z, Grinchuk V, Smith A, Qin B, Lu N, Wang D, Wang H, Ramalingam TR, Wynn TA, Urban JF Jr, Shea-Donohue T, Zhao A
Journal of immunology (Baltimore, Md. : 1950) 2015 Nov 15;195(10):4771-80
Journal of immunology (Baltimore, Md. : 1950) 2015 Nov 15;195(10):4771-80
Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.
Kan AA, de Jager W, de Wit M, Heijnen C, van Zuiden M, Ferrier C, van Rijen P, Gosselaar P, Hessel E, van Nieuwenhuizen O, de Graan PN
Journal of neuroinflammation 2012 Aug 30;9:207
Journal of neuroinflammation 2012 Aug 30;9:207
IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
Hvid M, Vestergaard C, Kemp K, Christensen GB, Deleuran B, Deleuran M
The Journal of investigative dermatology 2011 Jan;131(1):150-7
The Journal of investigative dermatology 2011 Jan;131(1):150-7
T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1579-84
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1579-84
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17E/IL-25 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB1258, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
