Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB13992 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17E/IL-25 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17E/IL-25 in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_542767
- Isotype
- IgG
- Antibody clone number
- 881014
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- CXCL1/GRO alpha Secretion Induced by IL-17E/IL-25 and Neutralization by Mouse IL-17E/IL-25 Antibody. Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL) induces CXCL1/GRO alpha secretion in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha Secretion elicited by Recombinant Mouse IL-17E/IL-25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB13992). The ND50 is typically 0.005-0.025 μg/mL.