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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
- Other assay [1]
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- Product number
- PA5-47107 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, and rhIL-17F is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization assay of recombinant human IL-17E/IL-25 induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the human CXCL1/GRO alpha Duoset. Under these conditions, CXCL1/GRO alpha secretion elicited by IL-17E/IL-25 is neutralized (green line) by increasing concentrations of Goat Anti-human IL-17E/IL-25 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47107). The ND50 is typically 0.04-0.24 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL-25 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in IL-25 polyclonal antibody (Product # PA5-47107). Recombinant Human IL‚17E/IL‚25 induces CXCL1/GRO alpha secretion in HT‚29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha Duoset. Under these conditions, CXCL1/GRO alpha secretion elicited by IL‚17E/IL‚25 is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚17E/IL‚25 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04-0.24 µg/mL.
