PA5-47107

antibody from Invitrogen Antibodies

Targeting: IL25 IL-17E, IL-25, IL17E

Blocking/Neutralizing (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47107

Provider

Invitrogen Antibodies

Product name

IL-25 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, and rhIL-17F is observed.

Concentration

0.2 mg/mL

Blocking/Neutralizing

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization assay of recombinant human IL-17E/IL-25 induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the human CXCL1/GRO alpha Duoset. Under these conditions, CXCL1/GRO alpha secretion elicited by IL-17E/IL-25 is neutralized (green line) by increasing concentrations of Goat Anti-human IL-17E/IL-25 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47107). The ND50 is typically 0.04-0.24 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of IL-25 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in IL-25 polyclonal antibody (Product # PA5-47107). Recombinant Human IL‚17E/IL‚25 induces CXCL1/GRO alpha secretion in HT‚29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha Duoset. Under these conditions, CXCL1/GRO alpha secretion elicited by IL‚17E/IL‚25 is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚17E/IL‚25 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04-0.24 µg/mL.