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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1258P - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17E/IL-25 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-17E in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse IL-17E and no cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, or rhIL-17F.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q9H293- Isotype
- IgG
- Antibody clone number
- 182203
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual γδT cells in early pregnancy.
A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
Zhang Y, Wang Y, Li MQ, Duan J, Fan DX, Jin LP
Experimental and therapeutic medicine 2018 Feb;15(2):1855-1862
Experimental and therapeutic medicine 2018 Feb;15(2):1855-1862
A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
Gironi M, Saresella M, Rovaris M, Vaghi M, Nemni R, Clerici M, Grossi E
Immunity & ageing : I & A 2013 Jan 10;10(1):1
Immunity & ageing : I & A 2013 Jan 10;10(1):1
IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
Hvid M, Vestergaard C, Kemp K, Christensen GB, Deleuran B, Deleuran M
The Journal of investigative dermatology 2011 Jan;131(1):150-7
The Journal of investigative dermatology 2011 Jan;131(1):150-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17E/IL-25 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human IL-17E/IL-25 PE-conjugated Monoclonal Antibody (Catalog # IC1258P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
