Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-2779-37 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD277 Monoclonal Antibody (eBioBT3.1 (20.1, BT3.1)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioBT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells. Applications Reported: This eBioBT3.1 (20.1, BT3.1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Applications Tested: This eBioBT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioBT3.1 (20.1, BT3.1)
- Vial size
- 2 mg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Butyrophilin-2A1 Directly Binds Germline-Encoded Regions of the Vγ9Vδ2 TCR and Is Essential for Phosphoantigen Sensing.
BTN3A2 expression in epithelial ovarian cancer is associated with higher tumor infiltrating T cells and a better prognosis.
CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells.
Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity.
Karunakaran MM, Willcox CR, Salim M, Paletta D, Fichtner AS, Noll A, Starick L, Nöhren A, Begley CR, Berwick KA, Chaleil RAG, Pitard V, Déchanet-Merville J, Bates PA, Kimmel B, Knowles TJ, Kunzmann V, Walter L, Jeeves M, Mohammed F, Willcox BE, Herrmann T
Immunity 2020 Mar 17;52(3):487-498.e6
Immunity 2020 Mar 17;52(3):487-498.e6
BTN3A2 expression in epithelial ovarian cancer is associated with higher tumor infiltrating T cells and a better prognosis.
Le Page C, Marineau A, Bonza PK, Rahimi K, Cyr L, Labouba I, Madore J, Delvoye N, Mes-Masson AM, Provencher DM, Cailhier JF
PloS one 2012;7(6):e38541
PloS one 2012;7(6):e38541
CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells.
Cubillos-Ruiz JR, Martinez D, Scarlett UK, Rutkowski MR, Nesbeth YC, Camposeco-Jacobs AL, Conejo-Garcia JR
Oncotarget 2010 Sep;1(5):329-38
Oncotarget 2010 Sep;1(5):329-38
Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity.
Yamashiro H, Yoshizaki S, Tadaki T, Egawa K, Seo N
Journal of leukocyte biology 2010 Oct;88(4):757-67
Journal of leukocyte biology 2010 Oct;88(4):757-67
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD277 Purified (filled histogram). Cells in the lymphocyte gate were used for analysis.