R31956

antibody from NSJ Bioreagents

Targeting: RNH1 RAI, RNH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31956

Provider

NSJ Bioreagents

Product name

Ribonuclease Inhibitor Antibody / RNH1

Antibody type

Polyclonal

Description

This highly specific Ribonuclease Inhibitor antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat, monkey samples.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Ribonuclease Inhibitor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis, 2) mouse brain, 3) human placenta and 4) human K562 lysate with Ribonuclease inhibitor antibody. Expected/observed molecular weight ~50 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain, 2) rat testis, 3) rat lung, 4) mouse brain, 5) mouse testis, 6) mouse lung and 7) mouse HEPA1-6 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human placenta, 2) human HeLa, 3) human SK-O-V3, 4) human Jurkat, 5) human U-87 MG, 6) monkey COS-7, 7) human SW620 and 8) human Caco-2 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A431 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.