TA501875

antibody from OriGene

Targeting: RNH1 RAI, RNH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501875

Provider

OriGene

Proper citation

OriGene Cat#TA501875, RRID:AB_11124610

Product name

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)

Antibody type

Monoclonal

Description

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

RNH1

Isotype

IgG

Antibody clone number

OTI3F11

Vial size

100 µl

Concentration

0.94 mg/ml

References

Submitted references

Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR.

Wang X, Teferedegne B, Shatzkes K, Tu W, Murata H
Analytical biochemistry 2016 Nov 15;513:21-27

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNH1 (RC208360, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNH1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNH1 monoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-RNH1 antibody(TA501875),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC208360 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNH1 antibody(TA501875), and then analyzed by flow cytometry.

Validation comment

FC