NBP1-36741

antibody from Novus Biologicals

Targeting: CNN2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-36741

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-36741, RRID:AB_2082133

Product name

Goat Polyclonal Calponin 2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_004359.1

Reactivity

Human

Host

Goat

Antigen sequence

internal region (near C Terminus)


Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Molecular cloning and characterization of human non-smooth muscle calponin.

Masuda H, Tanaka K, Takagi M, Ohgami K, Sakamaki T, Shibata N, Takahashi K
Journal of biochemistry 1996 Aug;120(2):415-24

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Molecular cloning and characterization of human non-smooth muscle calponin.

Masuda H, Tanaka K, Takagi M, Ohgami K, Sakamaki T, Shibata N, Takahashi K
Journal of biochemistry 1996 Aug;120(2):415-24

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Calponin 2 Antibody [NBP1-36741] - Analysis of Calponin 2 in HepG2 lysates (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calponin 2 Antibody [NBP1-36741] - (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.