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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-145463 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA5B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEMA5B in various lysates. Samples were incubated with SEMA5B polyclonal antibody (Product # PA5-145463) using a dilution of 2 μg/mL followed by HRP-Linked Caprine Anti-Rabbit IgG secondary antibody at a dilution of 0.2 µg/mL. Lane 1: Mouse Cerebrum lysate; Lane 2: Rat Cerebrum lysate; Lane 3: Human Serum.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SEMA5B in Human Cardiac Muscle Tissue. Samples were incubated in SEMA5B polyclonal antibody (Product # PA5-145463) using a dilution of 20 µg/mL followed by HRP-Linked Caprine Anti-Rabbit IgG at a dilution of 2 µg/mL and DAB staining.
