Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [13]
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Validation data
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- Product number
- NBP1-90081 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90081, RRID:AB_11002120
- Product name
- Rabbit Polyclonal Ropporin 1-like Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Ropporin 1-like antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects.
Onoufriadis A, Shoemark A, Schmidts M, Patel M, Jimenez G, Liu H, Thomas B, Dixon M, Hirst RA, Rutman A, Burgoyne T, Williams C, Scully J, Bolard F, Lafitte JJ, Beales PL, Hogg C, Yang P, Chung EM, Emes RD, O'Callaghan C, UK10K., Bouvagnet P, Mitchison HM
Human molecular genetics 2014 Jul 1;23(13):3362-74
Human molecular genetics 2014 Jul 1;23(13):3362-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ropporin 1-like Antibody [NBP1-90081] - Analysis in control (vector only transfected HEK293T lysate) and ROPN1L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human prostate shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human fallopian tube.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human bronchus, fallopian tube, liver and testis using Anti-ROPN1L antibody NBP1-90081 (A) shows similar protein distribution across tissues to independent antibody NBP2-47299 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human prostate shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining in human testis and prostate tissues using NBP1-90081 antibody. Corresponding ROPN1L RNA-seq data are presented for the same tissues.