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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP7764c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7764c, RRID:AB_2121036
- Product name
- HRAS Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Mutational analysis of HRAS and KRAS genes in oral carcinoma cell lines.
p66(Shc) restrains Ras hyperactivation and suppresses metastatic behavior.
Maemoto S, Yumoto M, Ibata M, Torizuka S, Ozawa N, Tatsumi S, Hashido M, Morikawa M, Maeda G, Imai K
Odontology / the Society of the Nippon Dental University 2012 Jul;100(2):149-55
Odontology / the Society of the Nippon Dental University 2012 Jul;100(2):149-55
p66(Shc) restrains Ras hyperactivation and suppresses metastatic behavior.
Ma Z, Liu Z, Wu RF, Terada LS
Oncogene 2010 Oct 14;29(41):5559-67
Oncogene 2010 Oct 14;29(41):5559-67
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-HRAS Antibody (Center) (Cat.#AP7764c) in Jurkat cell line lysates (35ug/lane). HRAS (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of HRAS Antibody (Center)(Cat#AP7764c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
