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- References [27]
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- Western blot [1]
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- Flow cytometry [1]
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- Product number
- 18295-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#18295-1-AP, RRID:AB_2121046
- Product name
- HRAS-Specific antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated HRAS-Specific antibody (Cat. #18295-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Validation of Isoform- and Mutation-Specific RAS Antibodies.
Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway.
Erianin, the main active ingredient of Dendrobium chrysotoxum Lindl, inhibits precancerous lesions of gastric cancer (PLGC) through suppression of the HRAS-PI3K-AKT signaling pathway as revealed by network pharmacology and in vitro experimental verification.
KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.
Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice.
Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.
Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.
H-Ras activation and fibroblast-induced TGF-β signaling promote laminin-332 accumulation and invasion in cutaneous squamous cell carcinoma.
A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.
Analysis of RAS protein interactions in living cells reveals a mechanism for pan-RAS depletion by membrane-targeted RAS binders.
Immunohistochemical assessment of growth factor signaling molecules: MAPK, Akt, and STAT3 pathways in oral epithelial precursor lesions and squamous cell carcinoma.
Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.
Cell-type specific tumorigenesis with Ras oncogenes in human lung epithelial cells.
Vertical Pathway Inhibition Overcomes Adaptive Feedback Resistance to KRASG12C Inhibition.
A systems mechanism for KRAS mutant allele-specific responses to targeted therapy.
KRAS regulation by small non-coding RNAs and SNARE proteins.
KRAS Oncoprotein Expression Is Regulated by a Self-Governing eIF5A-PEAK1 Feed-Forward Regulatory Loop.
The ERBB network facilitates KRAS-driven lung tumorigenesis.
Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.
Nogo-B receptor promotes epithelial-mesenchymal transition in non-small cell lung cancer cells through the Ras/ERK/Snail1 pathway.
Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.
Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.
Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.
MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B.
Paper-based microreactor array for rapid screening of cell signaling cascades.
Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells.
The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion.
Waters AM, Der CJ
Methods in molecular biology (Clifton, N.J.) 2021;2262:91-103
Methods in molecular biology (Clifton, N.J.) 2021;2262:91-103
Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway.
Zhang JJ, Song CG, Dai JM, Zhang XQ, Lin P, Li L, Yang XM, Chen ZN
American journal of translational research 2021;13(5):3967-3986
American journal of translational research 2021;13(5):3967-3986
Erianin, the main active ingredient of Dendrobium chrysotoxum Lindl, inhibits precancerous lesions of gastric cancer (PLGC) through suppression of the HRAS-PI3K-AKT signaling pathway as revealed by network pharmacology and in vitro experimental verification.
Wang Y, Chu F, Lin J, Li Y, Johnson N, Zhang J, Gai C, Su Z, Cheng H, Wang L, Ding X
Journal of ethnopharmacology 2021 Oct 28;279:114399
Journal of ethnopharmacology 2021 Oct 28;279:114399
KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.
Senoo H, Murata D, Wai M, Arai K, Iwata W, Sesaki H, Iijima M
Molecular cell 2021 Nov 18;81(22):4622-4634.e8
Molecular cell 2021 Nov 18;81(22):4622-4634.e8
Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice.
Jeong A, Cheng S, Zhong R, Bennett DA, Bergö MO, Li L
Acta neuropathologica communications 2021 Jul 27;9(1):129
Acta neuropathologica communications 2021 Jul 27;9(1):129
Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.
Liu C, Lu H, Wang H, Loo A, Zhang X, Yang G, Kowal C, Delach S, Wang Y, Goldoni S, Hastings WD, Wong K, Gao H, Meyer MJ, Moody SE, LaMarche MJ, Engelman JA, Williams JA, Hammerman PS, Abrams TJ, Mohseni M, Caponigro G, Hao HX
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Jan 1;27(1):342-354
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Jan 1;27(1):342-354
Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.
Röth S, Macartney TJ, Konopacka A, Chan KH, Zhou H, Queisser MA, Sapkota GP
Cell chemical biology 2020 Sep 17;27(9):1151-1163.e6
Cell chemical biology 2020 Sep 17;27(9):1151-1163.e6
H-Ras activation and fibroblast-induced TGF-β signaling promote laminin-332 accumulation and invasion in cutaneous squamous cell carcinoma.
Siljamäki E, Rappu P, Riihilä P, Nissinen L, Kähäri VM, Heino J
Matrix biology : journal of the International Society for Matrix Biology 2020 May;87:26-47
Matrix biology : journal of the International Society for Matrix Biology 2020 May;87:26-47
A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.
Bery N, Miller A, Rabbitts T
Nature communications 2020 Jun 26;11(1):3233
Nature communications 2020 Jun 26;11(1):3233
Analysis of RAS protein interactions in living cells reveals a mechanism for pan-RAS depletion by membrane-targeted RAS binders.
Li YC, Lytle NK, Gammon ST, Wang L, Hayes TK, Sutton MN, Bast RC Jr, Der CJ, Piwnica-Worms D, McCormick F, Wahl GM
Proceedings of the National Academy of Sciences of the United States of America 2020 Jun 2;117(22):12121-12130
Proceedings of the National Academy of Sciences of the United States of America 2020 Jun 2;117(22):12121-12130
Immunohistochemical assessment of growth factor signaling molecules: MAPK, Akt, and STAT3 pathways in oral epithelial precursor lesions and squamous cell carcinoma.
Tashiro K, Oikawa M, Miki Y, Takahashi T, Kumamoto H
Odontology 2020 Jan;108(1):91-101
Odontology 2020 Jan;108(1):91-101
Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.
Li M, Min W, Wang J, Wang L, Li Y, Zhou N, Yang Z, Qian Q
Experimental and therapeutic medicine 2020 Apr;19(4):2861-2870
Experimental and therapeutic medicine 2020 Apr;19(4):2861-2870
Cell-type specific tumorigenesis with Ras oncogenes in human lung epithelial cells.
Kumazaki M, Shimomura I, Kiyono T, Ochiya T, Yamamoto Y
Biochemical and biophysical research communications 2020 Apr 30;525(2):483-490
Biochemical and biophysical research communications 2020 Apr 30;525(2):483-490
Vertical Pathway Inhibition Overcomes Adaptive Feedback Resistance to KRASG12C Inhibition.
Ryan MB, Fece de la Cruz F, Phat S, Myers DT, Wong E, Shahzade HA, Hong CB, Corcoran RB
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 1;26(7):1633-1643
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 1;26(7):1633-1643
A systems mechanism for KRAS mutant allele-specific responses to targeted therapy.
McFall T, Diedrich JK, Mengistu M, Littlechild SL, Paskvan KV, Sisk-Hackworth L, Moresco JJ, Shaw AS, Stites EC
Science signaling 2019 Sep 24;12(600)
Science signaling 2019 Sep 24;12(600)
KRAS regulation by small non-coding RNAs and SNARE proteins.
Che Y, Siprashvili Z, Kovalski JR, Jiang T, Wozniak G, Elcavage L, Khavari PA
Nature communications 2019 Nov 11;10(1):5118
Nature communications 2019 Nov 11;10(1):5118
KRAS Oncoprotein Expression Is Regulated by a Self-Governing eIF5A-PEAK1 Feed-Forward Regulatory Loop.
Fujimura K, Wang H, Watson F, Klemke RL
Cancer research 2018 Mar 15;78(6):1444-1456
Cancer research 2018 Mar 15;78(6):1444-1456
The ERBB network facilitates KRAS-driven lung tumorigenesis.
Kruspig B, Monteverde T, Neidler S, Hock A, Kerr E, Nixon C, Clark W, Hedley A, Laing S, Coffelt SB, Le Quesne J, Dick C, Vousden KH, Martins CP, Murphy DJ
Science translational medicine 2018 Jun 20;10(446)
Science translational medicine 2018 Jun 20;10(446)
Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.
Luo C, Zhao S, Dai W, Zheng N, Wang J
Journal of agricultural and food chemistry 2018 Dec 12;66(49):13041-13049
Journal of agricultural and food chemistry 2018 Dec 12;66(49):13041-13049
Nogo-B receptor promotes epithelial-mesenchymal transition in non-small cell lung cancer cells through the Ras/ERK/Snail1 pathway.
Wu D, Zhao B, Qi X, Peng F, Fu H, Chi X, Miao QR, Shao S
Cancer letters 2018 Apr 1;418:135-146
Cancer letters 2018 Apr 1;418:135-146
Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.
Ross SJ, Revenko AS, Hanson LL, Ellston R, Staniszewska A, Whalley N, Pandey SK, Revill M, Rooney C, Buckett LK, Klein SK, Hudson K, Monia BP, Zinda M, Blakey DC, Lyne PD, Macleod AR
Science translational medicine 2017 Jun 14;9(394)
Science translational medicine 2017 Jun 14;9(394)
Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.
Wilson RHC, Coverley D
Scientific reports 2017 Jul 25;7(1):6475
Scientific reports 2017 Jul 25;7(1):6475
Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.
Liu W, Ning R, Chen RN, Huang XF, Dai QS, Hu JH, Wang YW, Wu LL, Xiong J, Hu G, Guo QL, Yang J, Wang H
Molecular carcinogenesis 2016 May;55(5):440-57
Molecular carcinogenesis 2016 May;55(5):440-57
MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B.
Park SJ, Shim JW, Park HS, Eum DY, Park MT, Mi Yi J, Choi SH, Kim SD, Son TG, Lu W, Kim ND, Yang K, Heo K
Oncogene 2016 Mar 10;35(10):1292-301
Oncogene 2016 Mar 10;35(10):1292-301
Paper-based microreactor array for rapid screening of cell signaling cascades.
Huang CH, Lei KF, Tsang NM
Lab on a chip 2016 Aug 7;16(15):2911-20
Lab on a chip 2016 Aug 7;16(15):2911-20
Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells.
Ceccarelli V, Nocentini G, Billi M, Racanicchi S, Riccardi C, Roberti R, Grignani F, Binaglia L, Vecchini A
PloS one 2014;9(1):e85025
PloS one 2014;9(1):e85025
The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion.
Cai WY, Wei TZ, Luo QC, Wu QW, Liu QF, Yang M, Ye GD, Wu JF, Chen YY, Sun GB, Liu YJ, Zhao WX, Zhang ZM, Li BA
Journal of cell science 2013 Jul 1;126(Pt 13):2877-89
Journal of cell science 2013 Jul 1;126(Pt 13):2877-89
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP( HRAS-Specific Antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 18295-1-AP( HRAS-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The HRAS-Specific antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human HRAS-Specific. This antibody recognizes human, mouse antigen. The HRAS-Specific antibody has been validated for the following applications: ELISA, IF, IHC, WB, FC analysis.
