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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-B10865 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ HRAS / H-Ras Antibody (aa111-189) LS-B10865
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: Hela, B: Hep G2 , C: Molt-4 . 12% SDS PAGE. HRAS / K-RAS antibody diluted at 1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- H-Ras antibody detects HRAS protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. H-Ras antibody dilution:1:1000
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using H-Ras antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using H-Ras antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using H-Ras antibody at 1:200 dilution.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-HRAS / H-Ras antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-HRAS / H-Ras antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
