- GTX116041 - GeneTex HRAS antibody

Submitted references Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells.

Gao P, Wang X, Jin Y, Hu W, Duan Y, Shi A, Du Y, Song D, Yang M, Li S, Han B, Zhao G, Zhang H, Fan Z, Miao QR
Breast cancer research : BCR 2018 Sep 12;20(1):112

Key Players of Cisplatin Resistance: Towards a Systems Pharmacology Approach.

Sarin N, Engel F, Rothweiler F, Cinatl J, Michaelis M, Frötschl R, Fröhlich H, Kalayda GV
International journal of molecular sciences 2018 Mar 7;19(3)

HRAS as a potential therapeutic target of salirasib RAS inhibitor in bladder cancer.

Sugita S, Enokida H, Yoshino H, Miyamoto K, Yonemori M, Sakaguchi T, Osako Y, Nakagawa M
International journal of oncology 2018 Aug;53(2):725-736

Oncogenic HrasG12V expression plus knockdown of Cdkn2a using ecotropic lentiviral vectors induces high-grade endometrial stromal sarcoma.

Brandt LP, Albers J, Hejhal T, Catalano A, Wild PJ, Frew IJ
PloS one 2017;12(10):e0186102

The transcription factor HOXB7 regulates ERK kinase activity and thereby stimulates the motility and invasiveness of pancreatic cancer cells.

Tsuboi M, Taniuchi K, Shimizu T, Saito M, Saibara T
The Journal of biological chemistry 2017 Oct 27;292(43):17681-17702

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: H-Ras antibody detects HRAS protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract12% SDS-PAGEH-Ras antibody (GTX116041) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: HeLa B: HepG2 (GTX27900) C: Molt-4 (GTX27912) 12% SDS PAGE GTX116041 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody (GTX116041) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with H-Ras antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with H-Ras antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras (GTX116041) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: H-Ras antibody detects H-Ras protein at membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. H-Ras antibody (GTX116041) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: H-Ras antibody detects H-Ras protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. H-Ras antibody (GTX116041) dilution: 1:500.

GTX116041

Antibody data