TA505674

antibody from OriGene

Targeting: HRAS HRAS1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505674

Provider

OriGene

Proper citation

OriGene Cat#TA505674, RRID:AB_2623274

Product name

HRAS mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Antibody type

Monoclonal

Description

HRAS mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

HRAS

Isotype

IgG

Antibody clone number

OTI1H3

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HRAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.

Validation comment

WB