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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C773436 - Provider product page
- Provider
- LSBio
- Product name
- HRAS / H-Ras Antibody (Atto 700) LS-C773436
- Antibody type
- Polyclonal
- Description
- Peptide Affinity Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Near infrared dye
- Storage
- Store at -20°C.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~21.3 kDa HRAS protein using Rabbit Anti-HRAS Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HRAS Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.3 kDa. Other Band(s): 25 kDa.
