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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8312 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nucleolar protein 2 Antibody / NOP2 / NSUN1
- Antibody type
- Polyclonal
- Description
- This highly specific Nucleolar protein 2 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Nucleolar protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEL, 2) Caco-2, 3) A549, 4) HeLa, 5) RT4, 6) 293T, 7) SiHa and 8) U-251 cell lysate with Nucleolar protein 2 antibody. Predicted molecular weight: 89 kDa but routinely observed at 100~120 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolar protein 2 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human RT4 cells with Nucleolar protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleolar protein 2 antibody.
