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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-92982 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHRS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLSAVARGYQ GWFHPCARLS VRMSSTGIDR KGVLANRVAV VTGSTSGIGF AIARRLARDG AHVVISSRKQ QNVDRAMAKL QGEGLSVAGI VCHVGKAEDR EQLVAKALEH CGGVDFLVCS AGVNPLVGST LGTSEQIWDK ILSVNVKSPA LLLSQLLPYM ENRRGAVILV SSIAAYNPVV ALGVYNVSKT ALLGLTRTLA LELAPKDIRV NCVVPGIIKT DFSKVVRIGF MGMSLSGRTS RNIISCRGLG SQRTVQESCP SCALQMPATS TGRTLRWQAT PLGSERSGGG CVAVVPGPGA; Positive Samples: HepG2, SKOV3, MCF7, BT-474, 22Rv1, Mouse liver, Mouse heart, Mouse bone marrow, Rat liver; Cellular Location: Mitochondrion matrix, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.12 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using DHRS2 Polyclonal antibody (Product # PA5-92982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DHRS2 in extracts of various cell lines using DHRS2 Polyclonal Antibody (Product # PA5-92982) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DHRS2 was performed in HeLa cells using DHRS2 Polyclonal Antibody (Product # PA5-92982).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DHRS2 in paraffin-embedded human breast cancer using DHRS2 Polyclonal Antibody (Product # PA5-92982) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DHRS2 in paraffin-embedded human uterine cancer using DHRS2 Polyclonal Antibody (Product # PA5-92982) at a dilution of 1:100.
