Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 15217-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15217-1-AP, RRID:AB_2299932
- Product name
- RPB5 antibody
- Antibody type
- Polyclonal
- Description
- RPB5 antibody (Cat. #15217-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.
Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity.
Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.
SPT5 stabilization of promoter-proximal RNA polymerase II.
RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.
Li Y, Huang J, Bao L, Zhu J, Duan W, Zheng H, Wang H, Jiang Y, Liu W, Zhang M, Yu Y, Yi C, Ji X
Molecular cell 2023 Apr 20;83(8):1280-1297.e11
Molecular cell 2023 Apr 20;83(8):1280-1297.e11
Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity.
Li Y, Huang J, Zhu J, Bao L, Wang H, Jiang Y, Tian K, Wang R, Zheng H, Duan W, Lai W, Yi X, Zhu Y, Guo T, Ji X
Molecular cell 2022 Oct 20;82(20):3943-3959.e11
Molecular cell 2022 Oct 20;82(20):3943-3959.e11
Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.
Jiang Y, Huang J, Tian K, Yi X, Zheng H, Zhu Y, Guo T, Ji X
Genome biology 2022 Nov 28;23(1):246
Genome biology 2022 Nov 28;23(1):246
SPT5 stabilization of promoter-proximal RNA polymerase II.
Aoi Y, Takahashi YH, Shah AP, Iwanaszko M, Rendleman EJ, Khan NH, Cho BK, Goo YA, Ganesan S, Kelleher NL, Shilatifard A
Molecular cell 2021 Nov 4;81(21):4413-4424.e5
Molecular cell 2021 Nov 4;81(21):4413-4424.e5
RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.
Zhang J, Pan YF, Ding ZW, Yang GZ, Tan YX, Yang C, Jiang TY, Liu LJ, Zhang B, Han T, Cao D, Yang T, Yang N, Wu MC, Dong LW, Wang HY
Oncogene 2015 Mar 19;34(12):1575-83
Oncogene 2015 Mar 19;34(12):1575-83
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15217-1-AP(POLR2E antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The RPB5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RPB5. This antibody recognizes human,mouse,rat antigen. The RPB5 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 15217-1-AP(POLR2E antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue