Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C742107 - Provider product page
- Provider
- LSBio
- Product name
- Mouse Anti-B4GALT1 Secondary Antibody (clone 11H3) LS-C742107
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 11H3
- Storage
- Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Independent validation of Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) with Mouse B4galt1 recombinant protein. Lane 1: 50 ng Mouse B4galt1 recombinant protein Lane 2: 25 ng Mouse B4galt1 recombinant protein Lane 3: 10 ng Mouse B4galt1 recombinant protein Lane 4: 50 ng Mouse B4galt1 recombinant protein Lane 5: 25 ng Mouse B4galt1 recombinant protein Lane 6: 10 ng Mouse B4galt1 recombinant protein Primary Antibody: Lane 1~3: Mouse B4galt1 Antibody (3C9) 1 µg/ml Lane 4~6: Mouse B4galt1 Antibody (11H3) (H&L) [IRDye8°°] (Licor,926-32211)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Independent validation of Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) with Mouse B4galt1 recombinant protein. Lane 1: 50 ng Mouse B4galt1 recombinant protein Lane 2: 25 ng Mouse B4galt1 recombinant protein Lane 3: 10 ng Mouse B4galt1 recombinant protein Lane 4: 50 ng Mouse B4galt1 recombinant protein Lane 5: 25 ng Mouse B4galt1 recombinant protein Lane 6: 10 ng Mouse B4galt1 recombinant protein Primary Antibody: Lane 1~3: Mouse B4galt1 Antibody (3C9) 1 µg/ml Lane 4~6: Mouse B4galt1 Antibody (11H3) (H&L) [IRDye8°°] (Licor,926-32211)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Standard curve of B4galt1 Sandwich ELISA. The B4galt1 Sandwich ELISA assay is developed by using Mouse B4galt1 Antibody (11H3) and Biotin conjugated Mouse B4galt1 Antibody (3C9) as capture and detect antibody, respectively. From the experimental results, Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) identify different epitopes. The sensitivity is 1-2 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA binding of Mouse B4galt1 Antibody (11H3) with Mouse B4galt1 recombinant protein. Coating antigen: B4galt1, 1 µg/ml. B4galt1 antibody dilutions start from 1000 ng/ml, EC50= 79.23 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Standard curve of B4galt1 Sandwich ELISA. The B4galt1 Sandwich ELISA assay is developed by using Mouse B4galt1 Antibody (11H3) and Biotin conjugated Mouse B4galt1 Antibody (3C9) as capture and detect antibody, respectively. From the experimental results, Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) identify different epitopes. The sensitivity is 1-2 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of Mouse B4galt1 Antibody (11H3) with Mouse B4galt1 recombinant protein. Coating antigen: B4galt1, 1 µg/ml. B4galt1 antibody dilutions start from 1000 ng/ml, EC50= 79.23 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Standard curve of B4galt1 Sandwich ELISA. The B4galt1 Sandwich ELISA assay is developed by using Mouse B4galt1 Antibody (11H3) and Biotin conjugated Mouse B4galt1 Antibody (3C9) as capture and detect antibody, respectively. From the experimental results, Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) identify different epitopes. The sensitivity is 1-2 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of Mouse B4galt1 Antibody (11H3) with Mouse B4galt1 recombinant protein. Coating antigen: B4galt1, 1 µg/ml. B4galt1 antibody dilutions start from 1000 ng/ml, EC50= 79.23 ng/ml.