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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-37939 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with human and cow based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of NSG1/NEEP21 in fetal mouse brain lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a NSG1/NEEP21 polyclonal antibody (Product # PA5-37939) at a dilution of 0.1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NSG1 in DIV9 cells using a NSG1 Monoclonal antibody (Product # PA5-37939) at 3 µg/mL. DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 staining in blue, Alexa 647) and not in the glia (GFAP staining in green, Alexa 488). Right panel shows the same cells in phase contrast.
