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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- RQ8428 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NEEP21 Antibody / Neuron-enriched endosomal protein of 21 kDa / NSG1
- Antibody type
- Polyclonal
- Description
- This highly specific NEEP21 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NEEP21 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SH-SY5Y, 2) rat brain and 3) mouse brain tissue lysate with NEEP21 antibody. Predicted molecular weight ~21 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human large B-cell lymphoma tissue with NEEP21 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human bladder urothelial carcinoma tissue with NEEP21 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with NEEP21 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human JK cells with NEEP21 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEEP21 antibody.
