Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS3854 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3854, RRID:AB_1914289
- Product name
- POLR3D (N314) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 281-330 of Human POLR3D.
- Description
- Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. POLR3D, also known as DNA-directed RNA polymerase III subunit RPC4 and Protein BN51, is a 398 amino acid nuclear protein that is one of the seventeen subunits that comprise Pol III. Specifically, POLR3D is a DNA-dependent RNA polymerase that uses the four ribonucleoside triphosphates to catalyze the transcription of DNA to RNA. The gene encoding POLR3D maps to human chromosome 8, which is made up of nearly 146 million bases and encodes about 800 genes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of POLR3D (N314) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:H9C2 whole cell lysate