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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 15339-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SRDLTLGGV KKKTFTPNII SRKIKEEPKE EVTVKKEKRE RDRDRQREGH GRGRGRPEVI QSHSIFEQGP AEMMKKKGNW DKTVDVSDMG PSHIINIKKE KRETDEETKQ ILRMLEKDDF LDDPGLRNDT RNMPVQLPLA HSGWLFKEEN DEPDVKPWLA GPKEEDMEVD IPAVKVKEEP RDEEEEAKMK APPKAARKTP GLPKDVSVAE LLRELSLTKE EELLFLQLPD TLPGQPPTQD IKPIKTEVQG EDGQVVLIKQ EKDREAKLAE NACTLADLTE GQVGKLLIRK SGRVQLLLGK VTLDVTMGTA CSFLQELVSV GLGDSRTGEM TVLGHVKHKL VCSPDFESLL DHKHR (45-398 aa encoded by B C004484)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 15339-1-AP (POLR3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-POLR3D (IP:15339-1-AP, 4ug; Detection:15339-1-AP 1:300) with HeLa cells lysate 2600 ug.
