12093-1-AP

antibody from Proteintech Group

Targeting: UBA5 FLJ23251, UBE1DC1

Provider product page for 12093-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [15]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

Submit

Product number: 12093-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12093-1-AP, RRID:AB_2211743
Product name: UBA5 antibody
Antibody type: Polyclonal
Description: UBA5 antibody (Cat. #12093-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

UBA5 protein structure - 12093-1-AP shown in red.

Submitted references A genus-wide interaction atlas across NS4B orthologues identifies a conserved role for UFMylation in orthoflavivirus replication.

Rajasekharan S, Barragan Torres VA, Pinheiro Gomes YC, Wilken L, Overhoff K, Lin YC, Patten SA, Watanabe S, Chatel-Chaix L, Scaturro P
Nature communications 2026 Mar 13;17(1)

The UFL1-AKT positive feedback loop promotes breast cancer progression by enhancing lipid synthesis.

Meng F, Du Y, Liang J, Li H, Wang J, Tang K, Kong R, Sun H, Yin T, Qin J, Yang X, Shan C, Liu M, Yang G, Zhang J, Wang Y, Zhou J, Chen Y
Nature communications 2026 Jan 20;17(1):614

UBA5 deficiency disrupts mitochondrial autophagy via the PINK1-parkin pathway and impairs myoblast proliferation.

Shi H, Cai Y, Liu Y
Biological research 2026 Feb 5;59(1)

Enhancement of MAVS UFMylation and inhibition of lysosomal degradation of MAVS aggregates: New findings on broad-spectrum antiviral mechanism of Anemoside B4.

Kang NX, Luo YM, Zhao JP, Jiang HL, Xu GQ, Cui NX, Deng KJ, Zhang L, Khan IA, Yang SL, Xu QM, Liu YL
Biochemical pharmacology 2025 Sep;239:117020

MCM5 UFMylation regulates replication origin firing and fork progression.

Li Z, Wu X, Liu L, Rao S, Liao Y, Liu M, Peng B, Zhang Q, Xia Y, Zhai Y, Takeda S, Xu X
The EMBO journal 2025 Nov;44(21):6019-6050

The UFMylation pathway is impaired in Alzheimer's disease.

Yan T, Heckman MG, Craver EC, Liu CC, Rawlinson BD, Wang X, Murray ME, Dickson DW, Ertekin-Taner N, Lou Z, Bu G, Springer W, Fiesel FC
bioRxiv : the preprint server for biology 2024 May 26;

UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER.

DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wąchalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature 2024 Mar;627(8003):445-452

UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity.

He C, Xing X, Chen HY, Gao M, Shi J, Xiang B, Xiao X, Sun Y, Yu H, Xu G, Yao Y, Xie Z, Xing Y, Budiarto BR, Chen SY, Gao Y, Lee YR, Zhang J
Molecular cell 2024 Mar 21;84(6):1120-1138.e8

The ufmylation cascade controls COPII recruitment, anterograde transport, and sorting of nascent GPCRs at ER.

Xu X, Huang W, Bryant CN, Dong Z, Li H, Wu G
Science advances 2024 Jun 21;10(25):eadm9216

The UFMylation pathway is impaired in Alzheimer's disease.

Yan T, Heckman MG, Craver EC, Liu CC, Rawlinson BD, Wang X, Murray ME, Dickson DW, Ertekin-Taner N, Lou Z, Bu G, Springer W, Fiesel FC
Molecular neurodegeneration 2024 Dec 18;19(1):97

Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice.

Jiajia H, Ziyao Y, Jiaqi Z, Yanli C, Xiaotao Z, Ming S
BMC cardiovascular disorders 2023 Nov 18;23(1):567

Ufmylation of UFBP1 Is Dispensable for Endoplasmic Reticulum Stress Response, Embryonic Development, and Cardiac and Intestinal Homeostasis.

Tandra V, Anderson T, Ayala JD, Weintraub NL, Singh N, Li H, Li J
Cells 2023 Jul 25;12(15)

ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.

Eck F, Phuyal S, Smith MD, Kaulich M, Wilkinson S, Farhan H, Behrends C
Journal of cell science 2020 Sep 16;133(18)

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.

Colin E, Daniel J, Ziegler A, Wakim J, Scrivo A, Haack TB, Khiati S, Denommé AS, Amati-Bonneau P, Charif M, Procaccio V, Reynier P, Aleck KA, Botto LD, Herper CL, Kaiser CS, Nabbout R, N'Guyen S, Mora-Lorca JA, Assmann B, Christ S, Meitinger T, Strom TM, Prokisch H, FREX Consortium, Miranda-Vizuete A, Hoffmann GF, Lenaers G, Bomont P, Liebau E, Bonneau D
American journal of human genetics 2016 Sep 1;99(3):695-703

Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.

Gavin JM, Hoar K, Xu Q, Ma J, Lin Y, Chen J, Chen W, Bruzzese FJ, Harrison S, Mallender WD, Bump NJ, Sintchak MD, Bence NF, Li P, Dick LR, Gould AE, Chen JJ
The Journal of biological chemistry 2014 Aug 15;289(33):22648-22658

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 12093-1-AP(UBA5 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using UBA5 antibody 12093-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-UBA5 (IP:12093-1-AP, 4ug; Detection:12093-1-AP 1:1000) with HeLa cells lysate 1800ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 12093-1-AP(UBA5 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue