A304-115A

antibody from Invitrogen Antibodies

Targeting: UBA5 FLJ23251, UBE1DC1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A304-115A

Provider

Invitrogen Antibodies

Product name

UBA5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human UBA5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBA5 antibody (Product # A304-115A; lot A304-115A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human UBA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-UBA5 antibody A304-115A (lot A304-115A-1) used for IP at 6 µg/mg lysate. UBA5 was also immunoprecipitated by rabbit anti-UBA5 antibody A304-114A. For blotting immunoprecipitated UBA5, A304-115A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human UBA5 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, 293T, and HeLa cells. Antibodies: Affinity purified rabbit anti-UBA5 antibody A304-115A (lot A304-115A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human UBA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBA5 antibody (Product # A304-115A; lot A304-115A-2) used for IP at 6 µg per reaction. UBA5 was also immunoprecipitated by a previous lot of this antibody (lot A304-115A-1). For blotting immunoprecipitated UBA5, Product # A304-115A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.