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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- MA5-22250 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCDHGA2 Monoclonal Antibody (2A7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: SGTSRICVKV LDANDNAPVF TQPEYRISIP ENTLVGTRIL TVTATDADEG YYAQVVYFLE KSPGETSEVF ELKSTSGELT IIKDLDYEDA TFHEIDIEAQ DGPGLLTRA
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCDHGA2 in cell lysate from Raw 264.7 cells, using a anti-PCDHGA2 monoclonal antibody (Product # MA5-22250).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCDHGA2 expressed in transfected 293T cell line by anti-PCDHGA2 monoclonal antibody (Product # MA5-22250), clone 2A7. Lane 1: PCDHGA2 transfected lysate (90.3 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCDHGA2 over-expressed 293 cell line, cotransfected with PCDHGA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDHGA2 monoclonal antibody, clone 2A7 (Product # MA5-22250). GAPDH (36.1 kDa) used as specificity and loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCDHGA2 using anti-PCDHGA2 monoclonal antibody (Product # MA5-22250) is approximately 0.1 ng/mL as a capture antibody.
