Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-59942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACAA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LLRGVFVVAA KRTPFGAYGG LLKDFTATDL SEFAAKAALS AGKVSPETVD SVIMGNVLQS SSDAIYLARH VGLRVGIPKE TPALTINRLC GSGFQSIVNG CQEICVKEAE VVLCGGTESM SQAPYCVRNV RFGTKLGSDI KLEDSLW Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACAA2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ACAA2 Polyclonal Antibody (Product # PA5-59942).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACAA2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ACAA2 Polyclonal Antibody (Product # PA5-59942).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ACAA2 in human cell line CACO-2 shows positivity in mitochondria. Samples were probed using an ACAA2 Polyclonal Antibody (Product # PA5-59942).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACAA2 in human kidney tissue shows strong cytoplasmic positivity in tubular cells. Samples were probed using an ACAA2 Polyclonal Antibody (Product # PA5-59942).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACAA2 in human kidney and pancreas tissues using ACAA2 Polyclonal Antibody (Product # PA5-59942). Corresponding ACAA2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACAA2 in human pancreas using ACAA2 Polyclonal Antibody (Product # PA5-59942) shows low expression as expected.