PA5-30409

antibody from Invitrogen Antibodies

Targeting: WASF3 KIAA0900, SCAR3, WAVE3

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30409

Provider

Invitrogen Antibodies

Product name

WASF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32.

Concentration

1 mg/mL

References

Submitted references

Secreted gelsolin desensitizes and induces apoptosis of infiltrated lymphocytes in prostate cancer.

Chen CC, Chiou SH, Yang CL, Chow KC, Lin TY, Chang HW, You WC, Huang HW, Chen CM, Chen NC, Chou FP, Chou MC
Oncotarget 2017 Sep 29;8(44):77152-77167

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using WASF3 Polyclonal Antibody (Product # PA5-30409). Sample (30 µg of whole cell lysate). Lane A: NT2D1 . 7.5% SDS PAGE. WASF3 Polyclonal Antibody (Product # PA5-30409) diluted at 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Intracellularly, sortilin binds to WASF3 and ATAD3A to promote the intake of cholesterol (A) Antibodies specific to sortilin, were used to co-precipitate ATAD3A and WASF3 from the cytoplasmic proteins of DU145 cells. (B) Various deletion mutants were constructed for ATAD3A and WASF3, which were respectively tagged with c - myc and human influenza hemagglutinin (HA), and the interactions between these proteins were examined. (C) Only full-length WASF3 co-precipitated with ATAD3A and sortilin (upper panel). ATAD3A did not co-precipitate with the deletion mutants DWN150 (without N-terminal 150 amino acid residues) and DWN220 of WASF3, although sortilin still co-precipitated with DWN150. The lower panel demonstrates the protein levels of the WASF3 mutants. (D) Wild-type ATAD3A co-precipitated with WASF3 and sortilin (upper panel). The deletion mutant DAN220 lost the ability to co-precipitate with WASF3 but not sortilin. The lower panel depicts the protein levels of ATAD3A. (E) Filipin III (FR4767, Sigma) fluorescent staining of cholesterol and lipoproteins. In control cells, filipin III fluorescence was detected from the plasma membrane to the nuclear envelope. The addition of secreted gelsolin (sGSN) increased the total amount of filipin III fluorescence, indicating that gelsolin increased the intake of cholesterol and lipoproteins. Knockdown of sortilin (SORT KD ), WASF-3 or ATAD3A reduced filipin III fluorescence. In WASF3 KD cells, though some filipin III staining wa