PA5-88756

antibody from Invitrogen Antibodies

Targeting: PDIA2 PDA2, PDI, PDIP, PDIR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88756

Provider

Invitrogen Antibodies

Product name

PDIA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FGLKAEAAPT LRLVNLETTK KYAPVDGGPV TAASITAFCH AVLNGQVKPY LLSQEIPPDW DQRPVKTLVG KNFEQVAFDE TKNVFVKFYA PWCTHCKEMA PAWEALAEKY QDHEDIIIAE LDATANELDA FAVHGFPTLK YFPAGPGRKV IEYKSTRDLE TFSKFLDNGG VLPTEEPPEE PAAPFPEPPA NSTMGSKEEL; Positive Samples: U-87MG, Mouse liver, Rat liver; Cellular Location: Endoplasmic reticulum lumen

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.64 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PDIA2 in extracts of various cell lines using PDIA2 Polyclonal Antibody (Product # PA5-88756) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PDIA2 was performed in NIH/3T3 cells using PDIA2 Polyclonal Antibody (Product # PA5-88756).