10209-2-AP

antibody from Proteintech Group

Targeting: CIRBP CIRP

Provider product page for 10209-2-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [76]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 10209-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10209-2-AP, RRID:AB_2080263
Product name: CIRBP antibody
Antibody type: Polyclonal
Description: KD/KO validated CIRBP antibody (Cat. #10209-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CIRBP protein structure - 10209-2-AP shown in red.

Submitted references Tat-CIRP Peptide Facilitates Frozen Wound Healing by Ameliorating Inflammation and Promoting Angiogenesis.

Li J, Ding J, Wu H, Lu C, Wu J, Luo Q
Journal of inflammation research 2024;17:2205-2215

Stellate Ganglion Block Attenuates LPS-Induced Acute Lung Injury by Activating Sirt3 Regulation of Oxidative Stress and Inflammation.

Dai S, Ji J, Li R, Gao L, He X
Biomedicines 2024 May 22;12(6)

The cold-inducible RNA-binding protein-Thioredoxin 1 pathway ameliorates mitochondrial dysfunction and mitochondrial dynamin-related protein 1 level in the hippocampus of aged mice with perioperative neurocognitive dysfunction.

Huang J, Zhu Y, Liu Y, Zhang R, Zhang Z, Liu J, Zhang Z, Liang Y, Ma B
CNS neuroscience & therapeutics 2024 Mar;30(3):e14433

SMYD5 is a regulator of the mild hypothermia response.

Rafnsdottir S, Jang K, Halldorsdottir ST, Vinod M, Tomasdottir A, Möller K, Halldorsdottir K, Reynisdottir T, Atladottir LH, Allison KE, Ostacolo K, He J, Zhang L, Northington FJ, Magnusdottir E, Chavez-Valdez R, Anderson KJ, Bjornsson HT
bioRxiv : the preprint server for biology 2024 Jul 12;

Radiation upregulates macrophage TREM-1 expression to exacerbate injury in mice.

Yamaga S, Murao A, Ma G, Brenner M, Aziz M, Wang P
Frontiers in immunology 2023;14:1151250

A Synthetic Poly(A) Tail Targeting Extracellular CIRP Inhibits Sepsis.

Murao A, Jha A, Ma G, Chaung W, Aziz M, Wang P
Journal of immunology (Baltimore, Md. : 1950) 2023 Oct 1;211(7):1144-1153

Chronic Kidney Disease Induces Proarrhythmic Remodeling.

King BMN, Mintz S, Lin X, Morley GE, Schlamp F, Khodadadi-Jamayran A, Fishman GI
Circulation. Arrhythmia and electrophysiology 2023 Jan;16(1):e011466

The new landscape of differentially expression proteins in placenta tissues of gestational diabetes based on iTRAQ proteomics.

Ge L, Huang P, Miao H, Yu H, Wu D, Chen F, Lin Y, Lin Y, Li W, Hua J
Placenta 2023 Jan;131:36-48

Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection.

Corre M, Boehm V, Besic V, Kurowska A, Viry A, Mohammad A, Sénamaud-Beaufort C, Thomas-Chollier M, Lebreton A
Nucleic acids research 2023 Dec 11;51(22):12459-12475

Resetting Proteostasis of CIRBP with ISRIB Suppresses Neural Stem Cell Apoptosis under Hypoxic Exposure.

Zou Y, Yuan Z, Sun Y, Zhai M, Tan Z, Guan R, Aschner M, Luo W, Zhang J
Oxidative medicine and cellular longevity 2022;2022:3627026

CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy.

Liu C, Cheng X, Xing J, Li J, Li Z, Jian D, Wang Y, Wang S, Li R, Zhang W, Shao D, Ma X, Chen X, Shen J, Shi C, Guo Z, Wang W, Fan T, Liu L, Tang H
International journal of biological sciences 2022;18(7):2882-2897

Necroptosis-Mediated eCIRP Release in Sepsis.

Reilly B, Tan C, Murao A, Nofi C, Jha A, Aziz M, Wang P
Journal of inflammation research 2022;15:4047-4059

Hepatic ER stress suppresses adipose browning through ATF4-CIRP-ANGPTL3 cascade.

Lv S, Zhou Y, Chen J, Yuan H, Zhang ZN, Luan B
Cell reports 2022 Sep 27;40(13):111422

Active Release of eCIRP via Gasdermin D Channels to Induce Inflammation in Sepsis.

Tan C, Reilly B, Jha A, Murao A, Lee Y, Brenner M, Aziz M, Wang P
Journal of immunology (Baltimore, Md. : 1950) 2022 May 1;208(9):2184-2195

Cold-inducible RNA-binding protein regulates cyclin B1 against spermatogenesis arrest caused by heat stress.

Liu H, Xu C, Bao M, Huang J, Zou L, Fan X, Zhu C, Xia W
Andrology 2022 Feb;10(2):392-403

Hypoxia causes mitochondrial dysfunction and brain memory disorder in a manner mediated by the reduction of Cirbp.

Liu Y, Xue C, Lu H, Zhou Y, Guan R, Wang J, Zhang Q, Ke T, Aschner M, Zhang W, Luo W
The Science of the total environment 2022 Feb 1;806(Pt 3):151228

CIRBP Knockdown Attenuates Tumourigenesis and Improves the Chemosensitivity of Pancreatic Cancer via the Downregulation of DYRK1B.

Chen X, Xie H, Wang X, Zheng Z, Jin S
Frontiers in cell and developmental biology 2021;9:667551

Exosome-Mediated eCIRP Release From Macrophages to Induce Inflammation in Sepsis.

Murao A, Tan C, Jha A, Wang P, Aziz M
Frontiers in pharmacology 2021;12:791648

Extracellular CIRP-Impaired Rab26 Restrains EPOR-Mediated Macrophage Polarization in Acute Lung Injury.

Zhang W, Wang Y, Li C, Xu Y, Wang X, Wu D, Gao Z, Qian H, You Z, Zhang Z, He B, Wang G
Frontiers in immunology 2021;12:768435

Cold-inducible RNA-binding protein migrates from the nucleus to the cytoplasm under cold stress in normal human bronchial epithelial cells via TRPM8-mediated mechanism.

Mao LP, Jiao Y, Xiang JH, Luo XW, He Q, Ran DH, Xu Q, Lang CH, Chen LX
Annals of translational medicine 2021 Sep;9(18):1470

Deficiency of cold-inducible RNA-binding protein exacerbated monocrotaline-induced pulmonary artery hypertension through Caveolin1 and CAVIN1.

Liu J, Ke X, Wang L, Zhang Y, Yang J
Journal of cellular and molecular medicine 2021 May;25(10):4732-4743

Normothermic machine perfusion attenuates hepatic ischaemia-reperfusion injury by inhibiting CIRP-mediated oxidative stress and mitochondrial fission.

Liu W, Fan Y, Ding H, Han D, Yan Y, Wu R, Lv Y, Zheng X
Journal of cellular and molecular medicine 2021 Dec;25(24):11310-11321

Cold-inducible RNA binding protein agonist enhances the cardioprotective effect of UW solution during extended heart preservation.

Pan HZ, Zhang LJ, Liu YW, Li YN, Su ZH, Meng J, Zhang H
Artificial organs 2020 Oct;44(10):E406-E418

Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R-mediated STAT3 activation.

Zhou M, Aziz M, Denning NL, Yen HT, Ma G, Wang P
JCI insight 2020 Mar 12;5(5)

Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis.

Denning NL, Aziz M, Murao A, Gurien SD, Ochani M, Prince JM, Wang P
JCI insight 2020 Mar 12;5(5)

A novel target to reduce microglial inflammation and neuronal damage after deep hypothermic circulatory arrest.

Liu M, Li Y, Gao S, Yan S, Zhang Q, Liu G, Ji B
The Journal of thoracic and cardiovascular surgery 2020 Jun;159(6):2431-2444.e7

Extracellular microRNA 130b-3p inhibits eCIRP-induced inflammation.

Gurien SD, Aziz M, Jin H, Wang H, He M, Al-Abed Y, Nicastro JM, Coppa GF, Wang P
EMBO reports 2020 Jan 7;21(1):e48075

Altered m(6)A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

Gokhale NS, McIntyre ABR, Mattocks MD, Holley CL, Lazear HM, Mason CE, Horner SM
Molecular cell 2020 Feb 6;77(3):542-555.e8

Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis.

Linders J, Madhi R, Rahman M, Mörgelin M, Regner S, Brenner M, Wang P, Thorlacius H
Laboratory investigation; a journal of technical methods and pathology 2020 Dec;100(12):1618-1630

The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.

Yan J, Goerne T, Zelmer A, Guzman R, Kapfhammer JP, Wellmann S, Zhu X
Frontiers in cell and developmental biology 2019;7:288

Loss of Cirbp expression is correlated with the malignant progression and poor prognosis in nasopharyngeal carcinoma.

Lin TY, Chen Y, Jia JS, Zhou C, Lian M, Wen YT, Li XY, Chen HW, Lin XL, Zhang XL, Xiao SJ, Sun Y, Xiao D
Cancer management and research 2019;11:6959-6969

Association between CIRP expression and hypoxic-ischemic brain injury in neonatal rats.

Chen L, Tian Q, Wang W
Experimental and therapeutic medicine 2019 Sep;18(3):1515-1520

CIRP downregulation renders cardiac cells prone to apoptosis in heart failure.

Chen M, Fu H, Zhang J, Huang H, Zhong P
Biochemical and biophysical research communications 2019 Oct 1;517(4):545-550

Cold-inducible RNA-binding protein as a novel target to alleviate blood-brain barrier damage induced by cardiopulmonary bypass.

Liu M, Li Y, Liu Y, Yan S, Liu G, Zhang Q, Ji B
The Journal of thoracic and cardiovascular surgery 2019 Mar;157(3):986-996.e5

Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia.

Downie Ruiz Velasco A, Welten SMJ, Goossens EAC, Quax PHA, Rappsilber J, Michlewski G, Nossent AY
Molecular therapy. Nucleic acids 2019 Mar 1;14:329-338

Therapeutic hypothermia protects photoreceptors through activating Cirbp pathway.

Sun YJ, Ma S, Fan B, Wang Y, Wang SR, Li GY
Neurochemistry international 2019 Jun;126:86-95

An annual cycle of gene regulation in the red-legged salamander mental gland: from hypertrophy to expression of rapidly evolving pheromones.

Wilburn DB, Feldhoff RC
BMC developmental biology 2019 Apr 27;19(1):10

Expression of cold-inducible RNA-binding protein (CIRP) in renal cell carcinoma and the effect of CIRP downregulation cell proliferation and chemosensitivity to gemcitabine.

Zhou KW, Jiang K, Zhu W, Weng G
Oncology letters 2018 May;15(5):7611-7616

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.

Ou J, Ball JM, Luan Y, Zhao T, Miyagishima KJ, Xu Y, Zhou H, Chen J, Merriman DK, Xie Z, Mallon BS, Li W
Cell 2018 May 3;173(4):851-863.e16

CIRP increases ICAM-1(+) phenotype of neutrophils exhibiting elevated iNOS and NETs in sepsis.

Ode Y, Aziz M, Wang P
Journal of leukocyte biology 2018 Apr;103(4):693-707

Cold-inducible RNA-binding protein (CIRP) induces translation of the cell-cycle inhibitor p27Kip1.

Roilo M, Kullmann MK, Hengst L
Nucleic acids research 2018 Apr 6;46(6):3198-3210

RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.

Pacheco NL, Heaven MR, Holt LM, Crossman DK, Boggio KJ, Shaffer SA, Flint DL, Olsen ML
Molecular autism 2017;8:56

Cold Inducible RNA Binding Protein Is Involved in Chronic Hypoxia Induced Neuron Apoptosis by Down-Regulating HIF-1α Expression and Regulated By microRNA-23a.

Chen X, Liu X, Li B, Zhang Q, Wang J, Zhang W, Luo W, Chen J
International journal of biological sciences 2017;13(4):518-531

Therapeutic Hypothermia Enhances Cold-Inducible RNA-Binding Protein Expression and Inhibits Mitochondrial Apoptosis in a Rat Model of Cardiac Arrest.

Wu L, Sun HL, Gao Y, Hui KL, Xu MM, Zhong H, Duan ML
Molecular neurobiology 2017 May;54(4):2697-2705

Involvement of Cold Inducible RNA-Binding Protein in Severe Hypoxia-Induced Growth Arrest of Neural Stem Cells In Vitro.

Zhang Q, Wang YZ, Zhang W, Chen X, Wang J, Chen J, Luo W
Molecular neurobiology 2017 Apr;54(3):2143-2153

Cold-inducible RNA-binding protein mediates airway inflammation and mucus hypersecretion through a post-transcriptional regulatory mechanism under cold stress.

Juan Y, Haiqiao W, Xie W, Huaping H, Zhong H, Xiangdong Z, Kolosov VP, Perelman JM
The international journal of biochemistry & cell biology 2016 Sep;78:335-348

Cold-inducible RNA-binding protein promotes epithelial-mesenchymal transition by activating ERK and p38 pathways.

Lee HN, Ahn SM, Jang HH
Biochemical and biophysical research communications 2016 Sep 2;477(4):1038-1044

Cold-inducible RNA-binding protein mediates cold air inducible airway mucin production through TLR4/NF-κB signaling pathway.

Chen L, Ran D, Xie W, Xu Q, Zhou X
International immunopharmacology 2016 Oct;39:48-56

Progranulin deficiency leads to severe inflammation, lung injury and cell death in a mouse model of endotoxic shock.

Yu Y, Xu X, Liu L, Mao S, Feng T, Lu Y, Cheng Y, Wang H, Zhao W, Tang W
Journal of cellular and molecular medicine 2016 Mar;20(3):506-17

Cold-inducible RNA binding protein in mouse mammary gland development.

Lujan DA, Garcia S, Vanderhoof J, Sifuentes J, Brandt Y, Wu Y, Guo X, Mitchell T, Howard T, Hathaway HJ, Hartley RS
Tissue & cell 2016 Dec;48(6):577-587

Cold-inducible RNA binding protein regulates mucin expression induced by cold temperatures in human airway epithelial cells.

Ran D, Chen L, Xie W, Xu Q, Han Z, Huang H, Zhou X
Archives of biochemistry and biophysics 2016 Aug 1;603:81-90

Cooling-induced SUMOylation of EXOSC10 down-regulates ribosome biogenesis.

Knight JR, Bastide A, Peretti D, Roobol A, Roobol J, Mallucci GR, Smales CM, Willis AE
RNA (New York, N.Y.) 2016 Apr;22(4):623-35

Cold-inducible RNA-binding protein inhibits neuron apoptosis through the suppression of mitochondrial apoptosis.

Zhang HT, Xue JH, Zhang ZW, Kong HB, Liu AJ, Li SC, Xu DG
Brain research 2015 Oct 5;1622:474-83

Analysis of gene expression profiles reveals the regulatory network of cold-inducible RNA-binding protein mediating the growth of BHK-21 cells.

Tang C, Wang Y, Lan D, Feng X, Zhu X, Nie P, Yue H
Cell biology international 2015 Jun;39(6):678-89

Hypothermic Preconditioning of Human Cortical Neurons Requires Proteostatic Priming.

Rzechorzek NM, Connick P, Patani R, Selvaraj BT, Chandran S
EBioMedicine 2015 Jun;2(6):528-35

Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.

Knight JR, Bastide A, Roobol A, Roobol J, Jackson TJ, Utami W, Barrett DA, Smales CM, Willis AE
The Biochemical journal 2015 Jan 15;465(2):227-38

RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.

Peretti D, Bastide A, Radford H, Verity N, Molloy C, Martin MG, Moreno JA, Steinert JR, Smith T, Dinsdale D, Willis AE, Mallucci GR
Nature 2015 Feb 12;518(7538):236-9

Cold-inducible RNA-binding protein, CIRP, inhibits DNA damage-induced apoptosis by regulating p53.

Lee HN, Ahn SM, Jang HH
Biochemical and biophysical research communications 2015 Aug 28;464(3):916-21

Cold-inducible RNA-binding protein mediates neuroinflammation in cerebral ischemia.

Zhou M, Yang WL, Ji Y, Qiang X, Wang P
Biochimica et biophysica acta 2014 Jul;1840(7):2253-61

Protein overexpression of CIRP and TLR4 in oral squamous cell carcinoma: an immunohistochemical and clinical correlation analysis.

Ren WH, Zhang LM, Liu HQ, Gao L, Chen C, Qiang C, Wang XL, Liu CY, Li SM, Huang C, Qi H, Zhi KQ
Medical oncology (Northwood, London, England) 2014 Aug;31(8):120

NF-κB-dependent role for cold-inducible RNA binding protein in regulating interleukin 1β.

Brochu C, Cabrita MA, Melanson BD, Hamill JD, Lau R, Pratt MA, McKay BC
PloS one 2013;8(2):e57426

Cold-inducible RNA-binding protein is an important mediator of alcohol-induced brain inflammation.

Rajayer SR, Jacob A, Yang WL, Zhou M, Chaung W, Wang P
PloS one 2013;8(11):e79430

The protective effect of Cold-inducible RNA-binding protein (CIRP) on testicular torsion/detorsion: an experimental study in mice.

Xia Z, Jiang K, Liu T, Zheng H, Liu X, Zheng X
Journal of pediatric surgery 2013 Oct;48(10):2140-7

Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.

Qiang X, Yang WL, Wu R, Zhou M, Jacob A, Dong W, Kuncewitch M, Ji Y, Yang H, Wang H, Fujita J, Nicastro J, Coppa GF, Tracey KJ, Wang P
Nature medicine 2013 Nov;19(11):1489-1495

Alternative promoters regulate cold inducible RNA-binding (CIRP) gene expression and enhance transgene expression in mammalian cells.

Al-Fageeh MB, Smales CM
Molecular biotechnology 2013 Jun;54(2):238-49

Effects of moderate and deep hypothermia on RNA-binding proteins RBM3 and CIRP expressions in murine hippocampal brain slices.

Tong G, Endersfelder S, Rosenthal LM, Wollersheim S, Sauer IM, Bührer C, Berger F, Schmitt KR
Brain research 2013 Apr 4;1504:74-84

Cold-inducible RNA-binding protein (CIRP) regulates target mRNA stabilization in the mouse testis.

Xia Z, Zheng X, Zheng H, Liu X, Yang Z, Wang X
FEBS letters 2012 Sep 21;586(19):3299-308

Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatogenic function in mouse testes.

Xia ZP, Zheng XM, Zheng H, Liu XJ, Liu GY, Wang XH
Asian journal of andrology 2012 Nov;14(6):884-9

Serum autoantibody signature of ductal carcinoma in situ progression to invasive breast cancer.

Mangé A, Lacombe J, Bascoul-Mollevi C, Jarlier M, Lamy PJ, Rouanet P, Maudelonde T, Solassol J
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Apr 1;18(7):1992-2000

Three-dimensional collagen represses cyclin E1 via β1 integrin in invasive breast cancer cells.

Wu Y, Guo X, Brandt Y, Hathaway HJ, Hartley RS
Breast cancer research and treatment 2011 Jun;127(2):397-406

Moderate low temperature preserves the stemness of neural stem cells and suppresses apoptosis of the cells via activation of the cold-inducible RNA binding protein.

Saito K, Fukuda N, Matsumoto T, Iribe Y, Tsunemi A, Kazama T, Yoshida-Noro C, Hayashi N
Brain research 2010 Oct 28;1358:20-9

Cold-inducible RNA-binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer.

Guo X, Wu Y, Hartley RS
Molecular carcinogenesis 2010 Feb;49(2):130-40

Down-regulating cold shock protein genes impairs cancer cell survival and enhances chemosensitivity.

Zeng Y, Kulkarni P, Inoue T, Getzenberg RH
Journal of cellular biochemistry 2009 May 1;107(1):179-88

Cold-inducible RNA binding protein (CIRP) expression is modulated by alternative mRNAs.

Al-Fageeh MB, Smales CM
RNA (New York, N.Y.) 2009 Jun;15(6):1164-76

Cold-inducible RNA-binding protein bypasses replicative senescence in primary cells through extracellular signal-regulated kinase 1 and 2 activation.

Artero-Castro A, Callejas FB, Castellvi J, Kondoh H, Carnero A, Fernández-Marcos PJ, Serrano M, Ramón y Cajal S, Lleonart ME
Molecular and cellular biology 2009 Apr;29(7):1855-68

Overexpression of CIRP may reduce testicular damage induced by cryptorchidism.

Zhou KW, Zheng XM, Yang ZW, Zhang L, Chen HD
Clinical and investigative medicine. Medecine clinique et experimentale 2009 Apr 1;32(2):E103-11

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP(CIRBP antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CIRBP antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CIRBP. This antibody recognizes human, mouse antigen. The CIRBP antibody has been validated for the following applications: ELISA, IHC, IP, WB, IF analysis.
Appendix: 10209-2-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-CIRBP (IP:10209-2-AP, 3ug; Detection:10209-2-AP 1:500) with HepG2 cells lysate 6000ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 10209-2-AP(CIRBP antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue