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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-31661 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIRBP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116. Predicted reactivity: Mouse (94%), Rat (94%), Chicken (94%), Rhesus Monkey (100%), Chimpanzee (96%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.67 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CIRBP Polyclonal Antibody (Product # PA5-31661). Sample (30 µg of whole cell lysate). Lane A: HCT116. 12% SDS PAGE. CIRBP Polyclonal Antibody (Product # PA5-31661) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CIRBP Polyclonal Antibody detects CIRBP protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIRBP protein stained by CIRBP Polyclonal Antibody (Product # PA5-31661) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
