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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF448 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-17RA/IL-17R Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-17 RA/IL-17 R in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL-17 RA/IL-17 R is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17 RD is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Population snapshots predict early haematopoietic and erythroid hierarchies.
IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity.
IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.
Functional interleukin-17 receptor A is expressed in central nervous system glia and upregulated in experimental autoimmune encephalomyelitis.
IL-17 receptor signaling influences virus-induced corneal inflammation.
Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi.
Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi.
Tusi BK, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M
Nature 2018 Mar 1;555(7694):54-60
Nature 2018 Mar 1;555(7694):54-60
IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity.
Subbarayal B, Chauhan SK, Di Zazzo A, Dana R
Journal of immunology (Baltimore, Md. : 1950) 2016 Nov 1;197(9):3464-3470
Journal of immunology (Baltimore, Md. : 1950) 2016 Nov 1;197(9):3464-3470
IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7398-407
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7398-407
TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):7119-30
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):7119-30
IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.
Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z
Cell research 2009 Feb;19(2):208-15
Cell research 2009 Feb;19(2):208-15
Functional interleukin-17 receptor A is expressed in central nervous system glia and upregulated in experimental autoimmune encephalomyelitis.
Das Sarma J, Ciric B, Marek R, Sadhukhan S, Caruso ML, Shafagh J, Fitzgerald DC, Shindler KS, Rostami A
Journal of neuroinflammation 2009 Apr 28;6:14
Journal of neuroinflammation 2009 Apr 28;6:14
IL-17 receptor signaling influences virus-induced corneal inflammation.
Molesworth-Kenyon SJ, Yin R, Oakes JE, Lausch RN
Journal of leukocyte biology 2008 Feb;83(2):401-8
Journal of leukocyte biology 2008 Feb;83(2):401-8
Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi.
Burchill MA, Nardelli DT, England DM, DeCoster DJ, Christopherson JA, Callister SM, Schell RF
Infection and immunity 2003 Jun;71(6):3437-42
Infection and immunity 2003 Jun;71(6):3437-42
Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi.
Burchill MA, Nardelli DT, England DM, DeCoster DJ, Christopherson JA, Callister SM, Schell RF
Infection and immunity 2003 Jun;71(6):3437-42
Infection and immunity 2003 Jun;71(6):3437-42
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IL-17 RA/IL-17 R by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF448) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-17 RA/IL-17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-17 RA/IL-17 R in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macro-phage cell line was stained with Goat Anti-Mouse IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF448, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
