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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB448 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17RA/IL-17R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17 RA/IL-17 R in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with recombinant human IL-17 RA/IL-17 R and no cross-reactivity with recombinant mouse IL-17B R.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q60943- Isotype
- IgG
- Antibody clone number
- 105828
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Production and functions of IL-17 in microglia.
Kawanokuchi J, Shimizu K, Nitta A, Yamada K, Mizuno T, Takeuchi H, Suzumura A
Journal of neuroimmunology 2008 Feb;194(1-2):54-61
Journal of neuroimmunology 2008 Feb;194(1-2):54-61
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse IL-17 RA/IL-17 R by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB448) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17 RA/IL-17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
