MAB4481

antibody from R&D Systems

Targeting: IL17RA CD217, CDw217, hIL-17R, IL-17RA, IL17R

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4481

Provider

R&D Systems

Product name

Mouse IL-17RA/IL-17R Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17 RA/IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross-reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q60943


Isotype

IgG

Antibody clone number

657603

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents.

Qiu AW, Liu QH, Wang JL
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(3):960-972

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Interleukin-17A Promotes Lung Tumor Progression through Neutrophil Attraction to Tumor Sites and Mediating Resistance to PD-1 Blockade.

Akbay EA, Koyama S, Liu Y, Dries R, Bufe LE, Silkes M, Alam MM, Magee DM, Jones R, Jinushi M, Kulkarni M, Carretero J, Wang X, Warner-Hatten T, Cavanaugh JD, Osa A, Kumanogoh A, Freeman GJ, Awad MM, Christiani DC, Bueno R, Hammerman PS, Dranoff G, Wong KK
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2017 Aug;12(8):1268-1279

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Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function.

Ippagunta SK, Gangwar R, Finkelstein D, Vogel P, Pelletier S, Gingras S, Redecke V, Häcker H
Proceedings of the National Academy of Sciences of the United States of America 2016 Oct 11;113(41):E6162-E6171

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Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.

Wu SY, Yu JS, Liu FT, Miaw SC, Wu-Hsieh BA
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3427-37

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-17 RA/IL-17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-6 secretion Induced by IL-17 and Neutralization by Mouse IL-17 RA/IL-17 R Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481). The ND₅₀ is typically 0.2-0.8 μg/mL.