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antibody from Invitrogen Antibodies
Targeting: IL17RA
CD217, CDw217, hIL-17R, IL-17RA, IL17R
Blocking/Neutralizing
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- 16-7917-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD217 (IL-17Ra) Monoclonal Antibody (424LTS), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 424LTS monoclonal antibody reacts with and blocks IL-17A binding to human interleukin 17 receptor A (IL-17RA, IL-17R). IL-17RA is a type I membrane protein of approximately 96 kDa (866 amino acids) and is involved in inflammatory responses to IL-17A, IL-17F and IL-17AF heterodimer. It forms heterodimeric complexes with several other members of its receptor family, including IL-17RC for IL-17A and IL-17F signaling and IL-17RC for IL-17E (IL-25) signaling. IL-17RA is ubiquitously expressed, but in leukocytes its expression is elevated on granulocytes and monocytes. Applications Reported: This 424LTS antibody has been reported for use in blocking IL-17 receptor A ligand binding. Applications Tested: The ND50 of 424LTS as measured by the inhibition of IL-6 induction in NHDF cells is 1-5 µg/mL in the presence of 3 ng/mL of recombinant Human IL-17A. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. This antibody should be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 424LTS
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression.
Crawford MP, Sinha S, Renavikar PS, Borcherding N, Karandikar NJ
Proceedings of the National Academy of Sciences of the United States of America 2020 Aug 11;117(32):19408-19414
Proceedings of the National Academy of Sciences of the United States of America 2020 Aug 11;117(32):19408-19414
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization of Human IL-17A Recombinant Protein activity by Anti-Human IL-17 Receptor A Functional Grade® Purified as measured by IL-6 induction in NHDF human fibroblast cells.
