46-7917-42
antibody from Invitrogen Antibodies
Targeting: IL17RA
CD217, CDw217, hIL-17R, IL-17RA, IL17R
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-7917-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD217 (IL-17Ra) Monoclonal Antibody (424LTS), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 424LTS monoclonal antibody reacts with and blocks IL-17A binding to human interleukin 17 receptor A (IL-17RA, IL-17R). IL-17RA is a type I membrane protein of approximately 96 kDa (866 amino acids) and is involved in inflammatory responses to IL-17A, IL-17F and IL-17AF heterodimer. It forms heterodimeric complexes with several other members of its receptor family, including IL-17RC for IL-17A and IL-17F signaling and IL-17RC for IL-17E (IL-25) signaling. IL-17RA is ubiquitously expressed, but in leukocytes its expression is elevated on granulocytes and monocytes. Applications Reported: This 424LTS antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 424LTS antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 424LTS
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Structure and signalling in the IL-17 receptor family.
Critical role of IL-17 receptor signaling in acute TNBS-induced colitis.
IL-17 receptor knockout mice have enhanced myelotoxicity and impaired hemopoietic recovery following gamma irradiation.
Cutting edge: interleukin 17 signals through a heteromeric receptor complex.
Gaffen SL
Nature reviews. Immunology 2009 Aug;9(8):556-67
Nature reviews. Immunology 2009 Aug;9(8):556-67
Critical role of IL-17 receptor signaling in acute TNBS-induced colitis.
Zhang Z, Zheng M, Bindas J, Schwarzenberger P, Kolls JK
Inflammatory bowel diseases 2006 May;12(5):382-8
Inflammatory bowel diseases 2006 May;12(5):382-8
IL-17 receptor knockout mice have enhanced myelotoxicity and impaired hemopoietic recovery following gamma irradiation.
Tan W, Huang W, Zhong Q, Schwarzenberger P
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):6186-93
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):6186-93
Cutting edge: interleukin 17 signals through a heteromeric receptor complex.
Toy D, Kugler D, Wolfson M, Vanden Bos T, Gurgel J, Derry J, Tocker J, Peschon J
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):36-9
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):36-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood was stained with Anti-Human CD4 FITC (Product # 11-0049-42), Anti-Human CD10 PE (Product # 12-0106-42) and Anti-Human CD217 (IL-17 Receptor A) PerCP-eFluor® 710. CD4 (hi) T cells (blue), CD4 (intermediate) monocytes (purple), and CD10+ granulocytes (orange) were gated (left) and analyzed for IL-17 Receptor A staining (right). The dotted line represents staining of Mouse IgG1 Isotype Control PerCP-eFluor® 710 from all cells.