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- Antibody Data
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- References [9]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF177 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17RA/IL-17R Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-17 RA/IL-17 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL-17 RA/IL-17 R is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q96F46- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A bi-specific inhibitor targeting IL-17A and MMP-9 reduces invasion and motility in MDA-MB-231 cells.
Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
Koslawsky D, Zaretsky M, Alcalay R, Mazor O, Aharoni A, Papo N
Oncotarget 2018 Jun 19;9(47):28500-28513
Oncotarget 2018 Jun 19;9(47):28500-28513
Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
Liu X, Jin H, Zhang G, Lin X, Chen C, Sun J, Zhang Y, Zhang Q, Yu J
PloS one 2014;9(9):e106834
PloS one 2014;9(9):e106834
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
International journal of cancer 2009 Sep 1;125(5):1092-101
International journal of cancer 2009 Sep 1;125(5):1092-101
Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4299-310
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4299-310
Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4299-310
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4299-310
The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2799-805
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2799-805
The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2799-805
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2799-805
IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3482-91
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3482-91
IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3482-91
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3482-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-17 RA/IL-17 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RA/IL-17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 secretion Induced by IL-17 and Neutralization by Human IL-17 RA/IL-17 R Antibody. Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL-6 Quantikine ELISA Kit (Catalog # D6050). IL-6 secretion elicited by Recombinant Human IL-17 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177). The ND50 is typically 0.1-0.5 µg/mL.
