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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-52715 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C12orf53 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DRSQKRRRPS GQQGALRQEE SQQPLTDLSP AGVTVLGAFG DSPTPTPDHE EPRGGPRPGM PHPKGAPAFQ LNR
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C12orf53 in control (vector only transfected HEK293T lysate) and PIANP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a C12orf53 Polyclonal Antibody (Product # PA5-52715).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of C12orf53 in human cell line SH-SY5Y using a C12orf53 Polyclonal Antibody (Product # PA5-52715) shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C12orf53 in human cerebral cortex and liver tissues using C12orf53 Polyclonal Antibody (Product # PA5-52715). Corresponding PIANP RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C12orf53 in human cerebral cortex using C12orf53 Polyclonal Antibody (Product # PA5-52715) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C12orf53 in human liver using C12orf53 Polyclonal Antibody (Product # PA5-52715) shows low expression as expected.
