PA5-27614

antibody from Invitrogen Antibodies

Targeting: TALDO1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27614

Provider

Invitrogen Antibodies

Product name

TALDO1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, mouse brain. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (80%), Pig (91%), Chicken (82%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

6.48 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The effects of fructose and metabolic inhibition on hepatocellular carcinoma.

Dewdney B, Alanazy M, Gillman R, Walker S, Wankell M, Qiao L, George J, Roberts A, Hebbard L
Scientific reports 2020 Oct 7;10(1):16769

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Evaluating A52 and Huh7 HCC growth and protein expression in glucose and fructose conditions. Cell proliferation via a BrdU assay was determined for ( a ) A52 cells, and ( b ) Huh7 cells grown in media containing 5 mM glucose or 5 mM fructose over 24, 48 and 72 h. Growth of ( c ) A52 and ( d ) Huh7 tumours was evaluated in mice fed a normal or high-fructose chow. Western blots of cell lysates for TKT, TALDO, PHGDH, G6PD, PSAT1 and beta-actin from, ( e ) A52 cells, and ( f ) Huh7 cells growth in glucose or fructose containing media for 24 and 48 h, and tumour lysates from ( g ) A52, and ( h ) Huh7 (H) subcutaneous tumours in mice fed a normal chow or high fructose diet. Original blots and densitometry analysis for the in vitro protein analysis and in vivo protein analysis are shown in Supplementary Figures 1 - 8 (Supplementary File 1 ). Full length western blot images for TKT, TALDO, PHGDH, PSAT1 and beta-actin could not be provided for ( e ) and ( g ). These images were provided from the thesis of M.A., and full-length original images could not be obtained. The original images in this figure and the Supplementary File are as presented in the thesis of M.A. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. G6PD glucose-6-phosphate dehydrogenase, PHGDH phosphoglycerate dehydrogenase, PSAT1 phosphoserine aminotransferase1, TALDO transaldolase, TKT transketolase.