HPA065699

antibody from Atlas Antibodies

Targeting: GEMIN4 DKFZP434B131, DKFZP434D174, HC56, HCAP1, HHRF-1, p97

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA065699

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA065699, RRID:AB_2685534

Product name

Anti-GEMIN4

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DLFFSVGNMIPTINHTILFELLKSLEASGLFIQLL
MALPTTICHAELERFLEHVTVDTSAEDVAFFLDVW
WEVMKHKGHPQDPLLSQFSAM


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines A-549 and SK-MEL-30 using Anti-GEMIN4 antibody. Corresponding GEMIN4 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A549 shows localization to nuclear bodies & cytosol.

Sample type

HUMAN