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LearnA300-867A
antibody from Invitrogen Antibodies
Targeting: GEMIN4
DKFZP434B131, DKFZP434D174, HC56, HCAP1, HHRF-1, p97
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A300-867A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Gemin4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Gemin4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Gemin4 antibody BL3554 (Cat. No. A300-867A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Gemin4 was also immunoprecipitated by rabbit anti-Gemin4 antibody BL3553, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Gemin4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 (Cat. No. A300-867A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
