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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-109979 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRPL28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPLHKYPVWL WKRLQLREGI CSRLPGHYLR SLEEERTPTP VHYRPHGAKF KINPKNGQRE RVEDVPIPIY FPPESQRGLW GGEGWILGQI YANNDKLSKR LKKVWKPQLF EREFYSEILD KKFTVTVTMR TLDLIDEAYG LDFYILKTPK EDLCSKFGMD LKRGMLLRLA RQDPQLHPED PERRAAIYDK YKEFAIPEEE AEWVGLTLEE AIEKQRLLEE KDPVPLFKIY VAELIQQLQQ QALSEPAVVQ KRASGQ
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MRPL28 in U2OS cells. Samples were incubated with MRPL28 Polyclonal antibody (Product # PA5-109979). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MRPL28 in 200 μg extracts of 293T cells. Samples were precipitated with 1 μg MRPL28 Polyclonal antibody (Product # PA5-109979). Western blot was performed from the immunoprecipitate using MRPL28 Polyclonal antibody (Product # PA5-109979) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MRPL28 in paraffin-embedded human gastric cancer. Samples were incubated with MRPL28 Polyclonal antibody (Product # PA5-109979) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MRPL28 in paraffin-embedded human lung cancer. Samples were incubated with MRPL28 Polyclonal antibody (Product # PA5-109979) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MRPL28 in paraffin-embedded rat ovary. Samples were incubated with MRPL28 Polyclonal antibody (Product # PA5-109979) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of MRPL28 in 293T cells. Samples were incubated in polyclonal MRPL28 antibody (Product # PA5-109979) using a dilution of 1:1000.
