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Learn12747-1-AP
antibody from Invitrogen Antibodies
Targeting: PPP2R2C
B55gamma, IMYPNO, MGC33570, PR52, PR55G
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- 12747-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP2R2C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LQVPVLKPM DLMVEVSPRR IFANGHTYHI NSISVNSDCE TYMSADDLRI NLWHLAITDR SFNIVDIKPA NMEDLTEVIT ASEFHPHHCN LFVYSSSKGS LRLCDMRAAA LCDKHSKLFE EPEDPSNRSF FSEIISSVSD VKFSHSGRYM LTRDYLTVKV WDLNMEARPI ETYQVHDYLR SKLCSLYEND CIFDKFECAW NGSDSVIMTG AYNNFFRMFD RNTKRDVTLE ASRESSKPRA VLKPRRVCVG GKRRRDDISV DSLDFTKKIL HTAWHPAENI IAIAATNNLY IFQDKVNSDM H (148-447 aa encoded by BC032954)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12747-1-AP ( PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug PPP2R2C antibody (12747-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
