Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00005721-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005721-M02, RRID:AB_2171087
- Product name
- Mouse Monoclonal PA28 Activator beta Subunit/PSME2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PSME2 - proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M02), clone 1G4.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - PSME2 monoclonal antibody (M02), clone 1G4 Analysis of PSME2 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Detection limit for recombinant GST tagged PSME2 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 transfected lysate using anti-PSME2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSME2 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of monoclonal antibody to PSME2 on formalin-fixed paraffin-embedded human stomach carcinoma. Antibody concentration 6 ug/ml.