Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-23954 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OLIG1 Monoclonal Antibody (257219)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Olig2 or rhOlig3. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 257219
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Chromosome Translocation t(14;21)(q11;q22) Activates Both OLIG1 and OLIG2 in Pediatric T-cell Lymphoblastic Malignancies and May Signify Adverse Prognosis.
Panagopoulos I, Gorunova L, Johannsdottir IMR, Andersen K, Holth A, Beiske K, Heim S
Cancer genomics & proteomics 2020 Jan-Feb;17(1):41-48
Cancer genomics & proteomics 2020 Jan-Feb;17(1):41-48
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Neuro-2A mouse neuroblastoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of human Olig1 Monoclonal Antibody (Product # MA5-23954) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OLIG1 in Neuro‚2A mouse neuroblastoma cell line and SH‚SY5Y human neuroblastoma cell line. Samples were incubated in OLIG1 monoclonal antibody (Product # MA5-23954) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of OLIG1 in immersion fixed paraffin-embedded sections of human glioma. Samples were incubated in OLIG1 monoclonal antibody (Product # MA5-23954) using a dilution of 8 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
