16616-1-AP

antibody from Proteintech Group

Targeting: LAG3 CD223

Provider product page for 16616-1-AP

Western blot

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Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [23]
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Validations
Western blot [1]

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Product number: 16616-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16616-1-AP, RRID:AB_2133350
Product name: LAG-3 antibody
Antibody type: Polyclonal
Description: LAG-3 antibody (Cat. #16616-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

LAG3 protein structure - 16616-1-AP shown in red.

Submitted references LAG-3-associated CD8(+) T-cell dysfunction in the cervical cancer tumor microenvironment.

Zhang G, Wang L, Chen J, Wang J, Ainiwaer A, Ma C
Frontiers in immunology 2026;17:1750726

Pan-cancer multi-omics profiling of gasdermins identifies GSDMD as an immune-related prognostic biomarker in kidney renal clear cell carcinoma.

Liu Y, Li X, Chen H, Mei N, Niu L, Li Y, Liu X, Jiang X, Zhang H
Discover oncology 2026 Mar 18;17(1)

Radiation-induced glioblastoma stem cell-mediated T cell exhaustion via EGR1-Gal3-LAG3 axis in glioblastoma.

Huang H, Li C, You H, Zhang Z, Lin Q, Yang J, Yu H, Li Z, Cui G, Song K, Zhang Q, Chen Y, Wang X, Zhang J, Gu D, Cheng C, Shao J
Cancer letters 2026 Jan 1;636:218125

Adiponectin attenuates atherosclerosis via macrophage polarization-mediated T Cell exhaustion by modulating the NF-κB p65/PI3K/Akt signaling pathway.

Qin M, Xu S, Chen Y, Luo X, Tang X, Zhang L, Xu Q
Tissue & cell 2026 Feb;98:103150

Activated T-cell membrane-derived nanocargoes displaying multi-immune checkpoints for enhanced cancer immunotherapy.

Du L, Zhang X, Gong Y, Liu M, Sun J, Hu X, Peng J, Liu Z, Zhang T, Xu J, Gao F, Cheng W
Materials today. Bio 2026 Feb;36:102702

Single-cell transcriptomics reveals hypoxia-driven iCAF_PLAU is associated with stemness and immunosuppression in anorectal malignant melanoma.

Zhang H, Gan L, Duan X, Tuo B, Zhang H, Liu S, Lian Y, Liu E, Sun Z
Journal of gastroenterology 2025 Oct;60(10):1242-1258

Peripheral nerve injury-induced remodeling of the tumor-associated macrophages promotes immune evasion in breast cancer.

Jiang Y, Zeng W, Feng Y, Deng X, Wang J, Liu H, Gao B, Bi D, Liu Z, Yang C, Chen M, Li T, Chen H, Zhang Y, Liang L, Xu J, Deng W, Yao Z, Wu W, Lao L, Chen J, Huang P
Journal of experimental & clinical cancer research : CR 2025 Oct 6;44(1):280

Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity.

Gu X, Gao C, Su X, Zhu Y, Fang Q, Yu J, Wang Z, Zhao D, Zhang W
Molecular cancer 2025 May 30;24(1):157

MIAT promotes tumor-infiltrating CD8(+) T-cell exhaustion and malignant progression of renal cell carcinoma via activating JAK3/STAT3 pathway.

Zhang MX, Jing LY, Tan HT, Dai ZR, Long DZ, Liu HC, Yu AZ, Wang B, Chen ZY, Luo JH, Chen ZH, Wang JF
Journal for immunotherapy of cancer 2025 Jul 31;13(7)

Helicobacter pylori CagA promotes gastric cancer immune escape by upregulating SQLE.

Liu S, Zhang N, Ji X, Yang S, Zhao Z, Li P
Cell death & disease 2025 Jan 14;16(1):17

PD-1 blockade does not improve efficacy of EpCAM-directed CAR T-cell in lung cancer brain metastasis.

Blobner J, Dengler L, Eberle C, Herold JJ, Xu T, Beck A, Mühlbauer A, Müller KJ, Teske N, Karschnia P, van den Heuvel D, Schallerer F, Ishikawa-Ankerhold H, Thon N, Tonn JC, Subklewe M, Kobold S, Harter PN, Buchholz VR, von Baumgarten L
Cancer immunology, immunotherapy : CII 2024 Oct 3;73(12):255

Implications of LAG3 and CTLA4 immune checkpoints beyond PD-1/PD-L1 as a potential target in determining the prognosis of uveal melanoma patients.

Kashyap S, Singh MK, Kumar N, Jha J, Lomi N, Meel R, Bakhshi S, Sen S, Singh L
The British journal of ophthalmology 2024 May 21;108(6):903-912

Single-cell combined bioinformatics analysis: construction of immune cluster and risk prognostic model in kidney renal clear cells based on CD8(+) T cell-associated genes.

Gao H, Sun H, He A, Liu H, Zhang Z, Li D, Mao W, Qian J
European journal of medical research 2024 Jan 30;29(1):89

The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning.

Zhu C, Du Y, Huai Q, Fang N, Xu W, Yang J, Li X, Zhang Y, Zhang X, Dai H, Li X, Wang H, Dai Y
Reproductive sciences (Thousand Oaks, Calif.) 2024 Feb;31(2):532-549

Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells.

Ma J, Yan S, Zhao Y, Yan H, Zhang Q, Li X
Frontiers in immunology 2023;14:1265255

Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells.

Sauer N, Szlasa W, Szewczyk A, Novickij V, Saczko J, Baczyńska D, Daczewska M, Kulbacka J
Pharmaceuticals (Basel, Switzerland) 2023 Sep 27;16(10)

Immune checkpoint B7-H3 is a therapeutic vulnerability in prostate cancer harboring PTEN and TP53 deficiencies.

Shi W, Wang Y, Zhao Y, Kim JJ, Li H, Meng C, Chen F, Zhang J, Mak DH, Van V, Leo J, St Croix B, Aparicio A, Zhao D
Science translational medicine 2023 May 10;15(695):eadf6724

Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma.

Lin M, He J, Zhang X, Sun X, Dong W, Zhang R, Xu Y, Lv L
The Journal of clinical investigation 2023 May 1;133(9)

Effect of DPP4/CD26 expression on SARS‑CoV‑2 susceptibility, immune response, adenosine (derivatives m(6)(2)A and CD) regulations on patients with cancer and healthy individuals.

Du J, Fu J, Zhang W, Zhang L, Chen H, Cheng J, He T, Fu J
International journal of oncology 2023 Mar;62(3)

CD4(+)LAG3(+)T cells are decreased in SSc-ILD and affect fibroblast mesenchymal transition by TGF-β3.

Qin L, Lin H, Zhu F, Wang J, Feng T, Xu T, Zhang G, Zhang X
iScience 2023 Dec 15;26(12):108225

The immune checkpoint expression in the tumor immune microenvironment of DLBCL: Clinicopathologic features and prognosis.

Ma J, Pang X, Li J, Zhang W, Cui W
Frontiers in oncology 2022;12:1069378

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Yang P, Wang K, Zhang C, Wang Z, Liu Q, Wang J, Jiang T, Shan X
Cancer immunology, immunotherapy : CII 2021 Sep;70(9):2589-2600

Single-cell RNA-seq reveals transcriptional landscape and intratumor heterogenicity in gallbladder cancer liver metastasis microenvironment.

Zhang Y, You WH, Li X, Wang P, Sha B, Liang Y, Qiu J, Zhou J, Hu H, Lu L
Annals of translational medicine 2021 May;9(10):889

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The LAG-3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human LAG-3. This antibody recognizes human,mouse,rat antigen. The LAG-3 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 16616-1-AP.pdf

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