Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB2319C - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal LAG-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAG-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Suppression of a broad spectrum of liver autoimmune pathologies by single peptide-MHC-based nanomedicines.
Umeshappa CS, Singha S, Blanco J, Shao K, Nanjundappa RH, Yamanouchi J, Parés A, Serra P, Yang Y, Santamaria P
Nature communications 2019 May 14;10(1):2150
Nature communications 2019 May 14;10(1):2150
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of LAG-3 in Human CD3+ PBMCs by Flow Cytometry. CD3+ human peripheral blood mononuclear cells (PBMCs) were either (A) untreated or (B) treated with 1 μg/mL PHA for 5 days were stained with Goat Anti-Human LAG-3 PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2319C) and Mouse Anti-Human CD3 epsilon Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on control antibody staining (Catalog # IC108C). View our protocol for Staining Membrane-associated Proteins.