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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 369304 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#369304, RRID:AB_2566480
- Product name
- Alexa Fluor® 647 anti-human CD223 (LAG-3)
- Antibody type
- Monoclonal
- Antigen
- Human LAG-3 transfected cells.
- Description
- CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antibody clone number
- 11C3C65
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- CD3/CD28/IL-2 stimulated (three days) peripheral blood monocular cells (PBMCs) were stained with CD8 FITC and CD223 (clone 11C3C65) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
- Conjugate
- Red dye
