Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 369206 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#369206, RRID:AB_2632656
- Product name
- PE anti-human CD223 (LAG-3)
- Antibody type
- Monoclonal
- Description
- CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 7H2C65
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- CD3/CD28/IL-2 stimulated (three days) peripheral blood monocular cells (PBMCs) were stained with CD8 Alexa Fluor® 647 and CD223 (clone 7H2C65) PE (top) or mouse IgG1, κ PE isotype control (bottom).
- Conjugate
- Yellow dye
